Part:BBa_K3072999:Design

pBAD + amajLime + His Expression Construct

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal NheI site found at 355
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BamHI site found at 239
  Illegal BamHI site found at 411
  Illegal XhoI site found at 420
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal AgeI site found at 74
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal SapI site found at 56
  

Design Notes

Inducible construct for controlled expression and strong RBS for ideally high yield. Polyhistidine tag allows for the purification of the expressed protein with affinity chromatography.

Source

Various